The frozen cartridge should be discarded, and a new one should be ordered. Also, the valve membranes will freeze and lose the ability to open and close, resulting in lane blockages. Sprint Cartridge: Improper storage of the sprint cartridgeīuffers in the cartridge will freeze if stored at -80☌. Prep Plates that reach room temperature during transit should be replaced and not returned to 4☌. Prep Plates will freeze at -20☌ or -80☌ and should be reordered if frozen due to damage to the magnetic beads. Prep plates: Improper storage of the Prep Plates Prolonged storage at -80☌ will damage the streptavidin binding surface, and a new cartridge should be ordered. If the Max/Flex Cartridge is briefly stored at -80C° (1 week or less), it can still be used without compromising its function. Max/Flex Cartridge: Improper storage of the Max/Flex cartridge This could introduce technical variance to the experiment thus, it is recommended to reorder a new CodeSet. If the CodeSet is stored at -20☌, it may display some loss in signal after 1-2 months.
If wanting to use a compromised codeset, it’s recommended to test with samples that have been run before on pristine codeset for comparison. Customers have gotten robust data from Codeset that was at RT for 8 hours, however these results are not guaranteed.
#Processing pmouse full
For example, if the -80☌ fails, the CodeSet will likely maintain full functionality after brief storage at 4☌ or even room temperature. Multiple F/T cycles can be detrimental to the CodeSet as they can damage RNA, DNA and fluorophore linkages however, CodeSet performance is generally robust and may not be dramatically affected. These reagents should be used at your own risk. NanoString cannot guarantee the performance of a component if it has not been stored at the recommended temperature. Reducing the input will ensure that the attenuation measurement will be accurate. If binding density is > 2, repeat the sample with ¼ of the RNA input. It is important that the un-attenuation sample counts be below the saturation threshold. Minimally, this test requires only half a cartridge to process. If attenuation is necessary, 90% will be a robust attenuating factor for almost any gene. In parallel, run the same sample with 90% attenuation by adding 180 pM of “cold” Reporter Probe oligo (Reporter Probe without the barcode) to the hybridization reaction containing 20 pM active Reporter Probe. If attenuation is necessary, the first step is to run at least one sample to determine the total raw counts for all genes (no attenuation). Attenuation is a strategy to reduce the number of over-abundant probe-target complexes bound to a cartridge and therefore increase detection of less-abundant targets. In essence, a highly overexpressed probe will occupy more of the available binding "real estate" on a cartridge. An over-abundance of one type of probe-target complex can reduce the chances that a low abundance target will be able to bind and be detected. The ROSALIND Platform for nCounter Analysisīecause capture and reporter probes are added in excess in each nCounter reaction, a highly overexpressed target gene may prevent the detection of low-abundance target by saturating the available surface area on a cartridge.AtoMx™ Spatial Informatics Platform Show submenu.CosMx SMI Overview – Single Cell Imaging.CosMx™ Spatial Molecular Imager Show submenu.nCounter Stem Cell Characterization Panel.
Stem Cells / Regenerative Medicine Show submenu.GeoMx® Digital Spatial Profiler Show submenu.Elevate your single-cell research with our new spatial multiomics instrument.